Super-Resolution Imaging Reveals the Nanoscale Distributions of Dystroglycan and Integrin Itga7 in Zebrafish Muscle Fibers

Cell signaling is determined partially by the localization and abundance of proteins. Dystroglycan integrin are both transmembrane receptors that connect cytoskeleton inside muscle cells to extracellular matrix outside cells, maintaining proper adhesion function muscle. The position relative integrins thought be important for function. subcellular quantification these receptor proteins can at nanometer scale FPALM super-resolution microscopy. We used determine localizations in fibers intact zebrafish (Danio rerio). Results were consistent with confocal imaging data, but illuminate further details nanoscale show feasibility using quantify interactions two a whole organism.